fixative fluid  (Servicebio Inc)

Journal: Frontiers in Immunology

Article Title: Single-cell sequencing decodes the secrets of the RAP phenomenon of corticotomy

doi: 10.3389/fimmu.2024.1397727

Figure Lengend Snippet: Pseudotime and SCENIC analyses revealed that Atf3 regulates a new direction for osteoclast differentiation (A) The monocytes_macrophages developmental trajectory was determined using Monocle. (B) A heat map of DEGs was made based on their simple kinetics through pseudotime using Dynverse and KEGG enrichment of each module. (C) The pseudotime expression trend diagram of osteoclast-related genes (Acp5, Ctsk, Mmp9, Dcstamp, Ocstamp, and Atp6v0d2) using pseudotime analysis; (D) A heat map of differentially expressed regulons in each monocyte_macrophage and the regulon specificity ranking diagram of each monocyte_macrophage. (E) ICC of CD68 and Atf3 and TRAP staining of osteoclasts in the control and corticotomy groups (white arrow indicates Atf3 and black arrow indicates the corresponding osteoclast).

Article Snippet: To perform TRAP staining, the samples were fixed with the TRAP fixed fluid for 1 min, and incubated with the TRAP liquid at 37°C for 50 min to stain osteoclasts, following the instructions provided with the kit (Solarbio, China).

Techniques: Expressing, Staining, Control

Journal: iScience

Article Title: Rosiglitazone retards the progression of iron overload-induced osteoarthritis by impeding chondrocyte ferroptosis

doi: 10.1016/j.isci.2024.110526

Figure Lengend Snippet:

Article Snippet: 4% Paraformaldehyde Fixative Tissue Fixation Fluid , Servicebio, Wuhan, China , G1101.

Techniques: Recombinant, Lysis, Glucose Assay, Western Blot, Staining, Bicinchoninic Acid Protein Assay, CCK-8 Assay, Isolation, Double Staining, GSSG Assay, Activity Assay, Membrane, Enzyme-linked Immunosorbent Assay, Software